Accidental microembolic signals: prevalence and clinical relevance
© Chen et al. 2016
Received: 12 December 2015
Accepted: 3 February 2016
Published: 12 April 2016
The purpose of this study was to examine the occurrence of accidental microembolic signals (MES) and its clinical relevance in patients receiving routine transcranial Doppler (TCD) examinations.
We retrospectively reviewed our institutional TCD database (from January 2007–November 2012). The arteries with positive MES, the presumed sources of emboli and the clinical backgrounds were analyzed.
A total of 10,067 patients received routine TCD examinations in our laboratory during the research period. MES were detected in 98 arteries of 78 patients, with a frequency of 0.77 % of all the recruited patients. A high percentage of MES (64.3 %) were detected in MCAs. Sixty five (83.33 %) accidental emboli were from arterial sources, including atherosclerotic cerebral or carotid artery stenosis (n = 45), moyamoya disease (n = 11), intracranial arteries (n = 3) and Takayasu arteritis (n = 3). Thirteen (16.67 %) emboli were from cardiac sources, including atrial fibrillation (n = 3), artificial valves (n = 8), infective endocarditis (n = 2), patent foramen ovale (n = 2), and systemic lupus erythematosus (n = 1). In artificial valves disease, all patients with MES were asymptomatic, while in atherosclerotic cerebral or carotid artery stenosis, 66.67 % (n = 30) patients with MES were symptomatic. In different diseases with accidental MES, the proportion of symptomatic patients and asymptomatic patients were different (p < 0.001).
MES are not uncommon during routine TCD examinations, the clinical value of which varied in different diseases.
KeywordsEmboli Microembolic signal Stroke Transcranial Doppler
Microembolic signals (MES) - the high-intensity transient signals detected by transcranial Doppler (TCD) - have been shown to correspond to microembolic materials within the cerebral arteries . MES can be detected in various clinical settings, such as carotid or intracranial stenosis, atrial fibrillation, and mechanical heart valves etc. Although most of these microemboli are clinically silent, many studies found that MES indicate increased risk of ischemic stroke, transient ischemic attack (TIA) or cognitive decline [2, 3].
According to the consensus on microembolus detection by TCD, the preferred recording time for patients with carotid stenosis or atrial fibrillation is at least 1 h . However, it is difficult to monitor the arteries for such a long time for each patient. During routine TCD examinations, MES can be detected occasionally. The occurrence and its clinical relevance have remained unknown. The goal of this study was to investigate accidental MES in a large TCD database.
Patients and methods
We retrospectively reviewed our institutional TCD database for hospitalized patients from January 2007–November 2012. During this period, the presence of accidental MES in routine TCD examinations was regularly recorded. All the patients in the database were unselectively recruited, including the patients from neurology, endocrine, cardiology and surgery departments. The reasons for TCD examinations varied, depending on the clinicians’ intention. According to the practice standards for TCD, the clinical indications included cerebral ischemia, intracranial arterial disease, detection of right-to-left shunts, sub-arachnoid hemorrhage, brain death, and periprocedural or surgical monitoring . All the recruited patients underwent a careful review of previous ischemic stroke and TIA history. Thorough evaluations were performed to determine the cause of stroke or TIA, including magnetic resonance image, carotid duplex, TCD, and electrocardiogram.
Ethics, consent and permissions
The study was approved by the Peking Union Medical College Hospital Research Ethics Committee. All the patients signed a consent form.
Transcranial doppler examination
Cerebral arteries were examined with 2-MHz Doppler instrument (Pioneer TC-8080 and Companion III, Nicolet-EME) according to standard protocol, including middle cerebral arteries (MCA), anterior cerebral arteries (ACA), posterior cerebral arteries (PCA), and C1 segment of the internal carotid arteries (ICA) through cranial temporal bone windows; ophthalmic arteries (OA) and ICA siphon through orbital windows; terminal vertebral arteries (VA) and basilar arteries (BA) through foraminal window . And carotid arteries were also insonated by using 4-MHz probe, including extracranial ICA, external carotid arteries (ECA) . All TCD examinations were performed by an experienced sonographer.
Microembolic signals detection
MES data were recorded and analyzed by an experienced observer, who was blinded to the clinical information. The following definitions for MES were used: typical visible and audible (click, chirp, whistle), short-duration, unidirectional, high-intensity signal (≥5 dB) within the Doppler flow spectrum with its occurrence at random in the cardiac cycle .
Student’s t-test was performed to compare continuous variables; Pearson χ2 and Fisher’s exact test were used to compare categorical variables between groups. A p value less than 0.05 was considered statistically significant. All statistical analyses were done with SPSS 16.0 (SPSS Inc).
During the study period, a total of 10,067 patients (5162 male, mean age 56.7 ± 17.4) received routine TCD examinations in our laboratory. Among them, 2305 (22.90 %) patients had previous ischemic events or TIA.
Arteries with microembolic signals
Sources of accidental emboli
Atherosclerotic cerebral or carotid artery stenosis
Patent foramen ovale
Systemic Lupus Erythematosus
In different diseases with accidental MES, the proportion of symptomatic patients and asymptomatic patients were different (Fisher exact test, p < 0.001). In artificial valves disease, all patients with MES were asymptomatic, while in atherosclerotic cerebral or carotid artery stenosis, 66.67 % (n = 30) patients with MES were symptomatic (Table 2).
In this study, accidental MES during routine TCD examinations were investigated in hospitalized patients with a large sample size. Overall, MES can be detected in 3 of 400 unselected hospitalized patients. A high percentage of MES (64.3 %) were detected in MCAs. It was not only because MCA is the common site of intracranial atherosclerosis and the largest artery that distributes blood to the cerebrum, but also the testing time of MCA was the longest, as the whole M1 segment could be detected by TCD.
The clinical significance of MES varied, as MES span a broad spectrum, ranging from harmless air bubbles to the large solid emboli . In patients with artificial valves, none of the 8 patients with MES had the symptoms of cerebral ischemia, probably because the air bubbles produced by artificial valves are harmless. In atherosclerotic cerebral or carotid artery stenosis, however, 66.67 % patients with MES were symptomatic. It has been confirmed that the presence of MES was an independent predictor of future stroke in atherosclerotic occlusive cerebrovascular diseases [2, 7]. According to the practice standards for TCD, in patients with symptomatic and asymptomatic extracranial or intracranial large artery disease, the MES information could be used to detect, localize, and quantify cerebral embolization, which may be helpful to establish the diagnosis and change management strategy . For example, one study demonstrated that in patients with MCA stenosis, the presence of an MES was associate with cerebral ischemia on diffusion weighted magnetic resonance imaging . Thus the diffusion weighted imaging procedure might be helpful for these patients to detect cerebral ischemia. As to the therapy strategy, two randomized clinical trials demonstrated that in patients with symptomatic carotid stenosis (≥50 %) and symptomatic cerebral or carotid artery stenosis, combination therapy with clopidogrel and aspirin was more effective than aspirin alone in reducing MES [10, 11]. Accidental MES in routine TCD examinations may also be an indicator for robust antiplatelet therapy.
It is interesting that MES were also observed in non-atherosclerotic artery stenosis including moyamoya disease, Takayasu’s arteritis, and intracranial arteries. It provide evidence that artery-to-artery embolism may also play an important role in the underlying mechanism of ischemic stroke in these patients .
This study was a single-center retrospective investigation. There was a possibility of selection bias and information bias.
In conclusion, our study suggests that MES are not uncommon during routine TCD examinations, the clinical value of which varied in different diseases. MES detection during routine TCD examinations may be of potential clinical value in the management of patients.
anterior cerebral arteries
external carotid arteries
internal carotid arteries
middle cerebral arteries
accidental microembolic signals
posterior cerebral arteries
transient ischemic attack
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- Spencer MP, Thomas GI, Nicholls SC, Sauvage LR. Detection of middle cerebral artery emboli during carotid endarterectomy using transcranial Doppler ultrasonography. Stroke. 1990;21:415–23.View ArticlePubMedGoogle Scholar
- Ritter MA. Prevalence and prognostic impact of microembolic signals in arterial sources of embolism. J Neurol. 2008;255:953–61.View ArticlePubMedGoogle Scholar
- Purandare N, Voshaar RC, Morris J, Byrne JE, Wren J, Heller RF, et al. Asymptomatic spontaneous cerebral emboli predict cognitive and functional decline in dementia. Biol Psychiatry. 2007;62:339–44.View ArticlePubMedGoogle Scholar
- Ringelstein EB, Droste DW, Babikian VL, Evans DH, Grosset DG, Kaps M, et al. Consensus on microembolus detection by TCD. International Consensus Group on Microembolus Detection. Stroke. 1998;29:725–9.View ArticlePubMedGoogle Scholar
- Alexandrov AV, Sloan MA, Tegeler CH, Newell DN, Lumsden A, Garami Z, et al. Practice standards for transcranial Doppler (TCD) ultrasound. Part II. Clinical indications and expected outcomes. J Neuroimaging. 2012;22:215–24.View ArticlePubMedGoogle Scholar
- Sloan MA, Alexandrov AV, Tegeler CH, Spencer MP, Caplan LR, Feldmann E, et al. Assessment: transcranial Doppler ultrasonography: report of the Therapeutics and Technology Assessment Subcommittee of the American Academy of Neurology. Neurology. 2004;62:468–81.View ArticleGoogle Scholar
- Gao S, Wong KS, Hansberg T, Lam WW, Droste DW, Ringelstein EB. Microembolic signal predicts recurrent cerebral ischemic events in acute stroke patients with middle cerebral artery stenosis. Stroke. 2004;35:2832–6.View ArticlePubMedGoogle Scholar
- Choi Y, Saqqur M, Stewart E, Stephenson C, Roy J, Boulanger JM, et al. Relative energy index of microembolic signal can predict malignant microemboli. Stroke. 2010;41:700–6.View ArticlePubMedGoogle Scholar
- Wong KS, Gao S, Chan YL, Hansberg T, Lam WW, Droste DW, et al. Mechanisms of acute cerebral infarctions in patients with middle cerebral artery stenosis: a diffusion-weighted imaging and microemboli monitoring study. Ann Neurol. 2002;52:74–81.View ArticlePubMedGoogle Scholar
- Markus HS, Droste DW, Kaps M, Larrue V, Lees KR, Siebler M, et al. Dual antiplatelet therapy with clopidogrel and aspirin in symptomatic carotid stenosis evaluated using doppler embolic signal detection: the Clopidogrel and Aspirin for Reduction of Emboli in Symptomatic Carotid Stenosis (CARESS) trial. Circulation. 2005;111:2233–40.View ArticlePubMedGoogle Scholar
- Wong KS, Chen C, Fu J, Chang HM, Suwanwela NC, Huang YN, et al. Clopidogrel plus aspirin versus aspirin alone for reducing embolisation in patients with acute symptomatic cerebral or carotid artery stenosis (CLAIR study): a randomised, open-label, blinded-endpoint trial. Lancet Neurol. 2010;9:489–97.View ArticlePubMedGoogle Scholar
- Chen J, Duan L, Xu WH, Han YQ, Cui LY, Gao S. Microembolic signals predict cerebral ischaemic events in patients with moyamoya disease. Eur J Neurol. 2014;21:785–90.View ArticlePubMedGoogle Scholar